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How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (Jul/24/2011 )

I've been searching and searching for literature on reasons why I didn't see Akt activation in response to VEGF stimulation in HUVECs. The only thing I can come up with is that I didn't serum starve the cells prior to stimulation. Growth factors in the serum activate pathways that are Akt dependent? That's my guess but I can't find anything to back it up. Any insight?


how do you run controls? how do you check activation of Akt?

-Inmost sun-