Stimulating hepatocytes with IL-1beta - (Apr/13/2010 )
I'm wondering if anyone knows of a generic concentration of IL-1beta that could be used in a stimulation experiment on hepatocytes? I am using Hep89 cells, and I want to stimulate in 6-well plates with estradiol-only and estradiol with IL-1beta. I wouldn't be sure on a concentration, no time to do a conc curve and papers only give values at 100U/ml which is no good to me as the protein I have is at 25ug/ml.
I don't know if you have seen this publication, maybe its helpful:
Le Vee M, Gripon P, Stieger B, Fardel O. Down-regulation of organic anion
transporter expression in human hepatocytes exposed to the proinflammatory
cytokine interleukin 1beta. Drug Metab Dispos. 2008 Feb;36(2):217-22. Epub 2007
Nov 8. PubMed PMID: 17991769
it seems that they have used 1ng/ml IL-1beta.