VEGF in cell nucleus - (Feb/12/2010 )
Have you ever read something about VEGF in cell nucleus?
Wich paper?
Does anyone have any good explanation for this phenomenon?
Regards
Hi,
This might help? They will at least have links to other references that might help too?
Subcellular localisation of VEGF in different pituitary cells. Changes of its expression in oestrogen induced prolactinomas
Authors: Mukdsi, Jorge; Paul, Ana; Gutiérrez, Silvina; Roth, Félix; Aoki, Agustín; Torres, Alicia1
Source: Journal of Molecular Histology, Volume 36, Numbers 8-9, October 2005 , pp. 447-454(8)
Abstract:
Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant feature demonstrated was the identification of this cytokine in the nucleus and cytoplasm of lactotrophs, somatotrophs and gonadotrophs, as well as in follicle-stellate cells of male rats. Oestrogen treatment increased the number of VEGF immunopositive cells and its expression detected differentially by western blot in both nucleus and cytoplasm of pituitary cells when compared to the control. At ultrastructural level VEGF appeared associated with nucleolus and euchromatin involving a possible internal autocrine loop. In lactotrophs, the predominant cell of the tumour, VEGF was immunodetected in RER, Golgi complex, and vesicular organelles, supporting further the association with an auto-paracrine effect exerted by VEGF. The nucleus/cytoplasm ratio of VEGF revealed a prevalent accumulation of VEGF in the cytoplasm. The presence of VEGF in the nucleus may probably be associated with a translocation to this cell compartment. This study demonstrated a cytoplasmic and nuclear immunolocalisation of VEGF in normal and tumoural adenohypophyseal cells. In the course of prolactinoma development, the oestrogen stimulated VEGF expression in tumoural cells, promoting a vascular adaptation which contributes to growth and progression of the tumour.
Thanks a lot DrAnt1, sorry the delay.
It's really helpfull
I will try to get this paper for a better analysis.
Regards
DrAnt1 on Feb 14 2010, 11:16 PM said:
This might help? They will at least have links to other references that might help too?
Subcellular localisation of VEGF in different pituitary cells. Changes of its expression in oestrogen induced prolactinomas
Authors: Mukdsi, Jorge; Paul, Ana; Gutiérrez, Silvina; Roth, Félix; Aoki, Agustín; Torres, Alicia1
Source: Journal of Molecular Histology, Volume 36, Numbers 8-9, October 2005 , pp. 447-454(8)
Abstract:
Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant feature demonstrated was the identification of this cytokine in the nucleus and cytoplasm of lactotrophs, somatotrophs and gonadotrophs, as well as in follicle-stellate cells of male rats. Oestrogen treatment increased the number of VEGF immunopositive cells and its expression detected differentially by western blot in both nucleus and cytoplasm of pituitary cells when compared to the control. At ultrastructural level VEGF appeared associated with nucleolus and euchromatin involving a possible internal autocrine loop. In lactotrophs, the predominant cell of the tumour, VEGF was immunodetected in RER, Golgi complex, and vesicular organelles, supporting further the association with an auto-paracrine effect exerted by VEGF. The nucleus/cytoplasm ratio of VEGF revealed a prevalent accumulation of VEGF in the cytoplasm. The presence of VEGF in the nucleus may probably be associated with a translocation to this cell compartment. This study demonstrated a cytoplasmic and nuclear immunolocalisation of VEGF in normal and tumoural adenohypophyseal cells. In the course of prolactinoma development, the oestrogen stimulated VEGF expression in tumoural cells, promoting a vascular adaptation which contributes to growth and progression of the tumour.