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Cytokines feeding cell culture - (Sep/14/2013 )

Hi everybody,

I need to add cytokines to my cell cultures and in some protocols say I should add certain amount of cytokines per number of cells and in other protocols only refer to cytokines concentration in the culture.

If I consider cytokine concentration it looks it is irrelevant the cell number I have in the culture. But maybe for stimulation with cytokines it is in fact important the amount of cytokines per number of cells.

I would appreciate your comments! thanks!


Usually no of cells seeded per well depend on the surface area of the well; I ue 1e5 per cm2; the vol of media depends on the duration of the culture; finally, amount of cytokine usually depends on the vol of media added.