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transfection in floating cells - (Jun/25/2009 )

Hi,

I wanted to transfect U937 cells. If any body has same experience, please share the technique and protocol with me.

-bhawana-

I have never worked with such cells, but I did transfect 293T cells while they were detached from the plates. I think you could go with your normal transfection protocol. Moreover, if you use products such as Fugene, i'm pretty sure you could find the infos on the data sheet.

-madrius1-

madrius1 on Jun 25 2009, 08:27 PM said:

I have never worked with such cells, but I did transfect 293T cells while they were detached from the plates. I think you could go with your normal transfection protocol. Moreover, if you use products such as Fugene, i'm pretty sure you could find the infos on the data sheet.




Hi,

Thanks for reply,
I have done a lot of successful transfection in adherent cell lines, but when ever I tried in floating cells, I have failed. I did electroporation also but I didnt work. Now I want to order some transfection reagent. I want to make sure that which will be better either Fugene or nucleufectin, or other available agents. If you have any experience, please let me know.

-bhawana-

bhawana on Jun 25 2009, 09:39 PM said:

madrius1 on Jun 25 2009, 08:27 PM said:

I have never worked with such cells, but I did transfect 293T cells while they were detached from the plates. I think you could go with your normal transfection protocol. Moreover, if you use products such as Fugene, i'm pretty sure you could find the infos on the data sheet.




Hi,

Thanks for reply,
I have done a lot of successful transfection in adherent cell lines, but when ever I tried in floating cells, I have failed. I did electroporation also but I didnt work. Now I want to order some transfection reagent. I want to make sure that which will be better either Fugene or nucleufectin, or other available agents. If you have any experience, please let me know.


Hi,

My PhD, who guides me in these researches works with U937, and i'm sure she uses FuGene (Roche). As soon as possible i will reply you on. if you want you may send me mail to: vinicius.biomed@gmail.com

Regards

Reis,V.P.

-Richard.21-

Hi,

Thanks.
As sson as you get this information please let me know



Reis.V.P on Jun 28 2009, 04:52 AM said:

bhawana on Jun 25 2009, 09:39 PM said:

madrius1 on Jun 25 2009, 08:27 PM said:

I have never worked with such cells, but I did transfect 293T cells while they were detached from the plates. I think you could go with your normal transfection protocol. Moreover, if you use products such as Fugene, i'm pretty sure you could find the infos on the data sheet.




Hi,

Thanks for reply,
I have done a lot of successful transfection in adherent cell lines, but when ever I tried in floating cells, I have failed. I did electroporation also but I didnt work. Now I want to order some transfection reagent. I want to make sure that which will be better either Fugene or nucleufectin, or other available agents. If you have any experience, please let me know.


Hi,

My PhD, who guides me in these researches works with U937, and i'm sure she uses FuGene (Roche). As soon as possible i will reply you on. if you want you may send me mail to: vinicius.biomed@gmail.com

Regards

Reis,V.P.

-bhawana-