Protocol Online logo
Top : New Forum Archives (2009-): : Tissue and Cell Culture

Splitting or feeding cells for the weekend - (Dec/02/2011 )

Is there a generally accepted rule of thumb for how to feed cells for a weekend? If you normally feed every 2 days can you just give them more media on Friday (and how much more)? Does it make a difference if there are or aren't growth factors in your media for whatever cell types you're working on at the time?



It will depend on the cell line, but I usually find that most of the common cell lines will easily last over the weekend on whatever medium they are in. Adding more medium would work to some extent, though often it is not about adding fresh nutrients, but removing waste products.

Some growth factors are very labile, so they may well degrade over a day or so.