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Nucleofection of ES cells, medium instead of nucleofector solution? - (Jun/15/2010 )

In our lab we have Human Cancer Stem Cells derived of pacients and I need to introduce a forgein DNA into them, but all methods fail to tranfect primary cells. So, I will use the Nucleofector Technology, but I have not the expensive nucleofector solution...
I would like to know how I can replace the expensive Nuclefector solution to electroporate my ES cells. Can I use cell culture medium of Cancer Stem Cells?

thanks in advance.


cultre medium doent work with nucleofectors, try some transfecting reagents (without use of neuclefectortm) like nanojuice,. lipofectamin, they reduced the prices for it and also comes a free sample kits for testing
good luck