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Generation of single cell suspensions from neural tissue - (Dec/10/2012 )

Hey guys, just wondering if anyone has a buffer/enzyme mix they use to reduce cell death when generating a single cell suspension from neuronal tissue.

I'm looking to run some flow cytometry with them after isolation.

Thanks, Jacob

-JacobThomas-

anyone?

-JacobThomas-

Have you tried Accutase ?

-Tabaluga-