stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (Dec/01/2009 )
i need advice from every one who has exprince about cytokines stored after collection from supernatant of cell culture(monocytes) untill mesaurment by ELISA.
can i store the supernatant which contain cytokines at -80 oC or -20 oC?
-20 will be ok!
-20 should be fine. However, be very careful to minimize freeze thaws or your cytokines will crap out!