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Mitochondria extraction from human liver cells - (Feb/25/2010 )

Hi Folks,

Any one can help me finding the best method of extracting mitochondria from human liver cells without damaging the membrane bound enzymes (outter and inner membrane bound).

-Mr. Naive-


have a look at a mitochondria isolation kit, like from Qiagen. If you need further purification you can add up a gradient centrifugation. Additionally check the publications from H. Shaegger. His group is doing that frequently.