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Transfecting three plasmids into hES cells - (Jun/28/2013 )


I would like to transiently transfect 3 different plasmid DNAs into human embryonic stem cells by lipofectamine. If cytotoxicity is not a huge issue, what amount of each plasmid would you use in a well of 6-well plate.

any suggestion is appreciated.



I think what matters is the total amount of plasmids transfected. You can use a high dose that does not cause huge cytotoxicity.


2 ug for each plasmid, and 6 ug in total