Where to obtain rat embryonic stem cells? - (Nov/04/2012 )
May i know if there is any way i can obtain rat embryonic stem cells?
I would like to use the rat embryonic stem cells to be used as my positive control in my RT-PCR, to obtained the Oct4 band.
Usually rat embryo is a good option. Or buy a cell line.
But since you actually need only RNA and as a control, probably buying rat embryo RNA of certain age would be more affordable.
Like here, but that's just first search result, probably there are more and some supliers may even have RNA directly from rat ESC line.
Thank you very much for the suggestion and link.
But i hope my supervisor is willing to buy ~
Or you can go hunting into sewers
(or pet shop and buy a pregnant rat, but that may have etical problems and also you would be required to have an animal handling certificate.. and also, really citing this kind of control RNA source would be.. pretty dificult)