Death of cells in the control. - (Dec/15/2010 )
I am doing toxicity experiments,but I have a problem. I usually add my drug to the cells and icubate it for a certain time, then measure the absorbance and compare it with the control, which has no drug in it. But I can see that some of the cells of my control are dead as well, I can see some holes, which means some of the cells have come off. Do you know why this might be?
You normally get some dead cells in any cell population - this is the reason you have controls... to show that the level of death (in your case) is greater with treatment than without.