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Compare ChIP to cell lysate - (Apr/15/2011 )

I am doing a chromatin immunoprecipitation and I would like to compare the amount of protein I purify with my antibody to the amount of the same protein left over in my cell lysate. I can't use a loading control because the ChIP will only have my protein of interest and nothing else to compare it to.

Thank You

-mcb56-

Did you figure out what type of control was appropriate? I'm curious too.

-ssmemo-