Compare ChIP to cell lysate - (Apr/15/2011 )
I am doing a chromatin immunoprecipitation and I would like to compare the amount of protein I purify with my antibody to the amount of the same protein left over in my cell lysate. I can't use a loading control because the ChIP will only have my protein of interest and nothing else to compare it to.
Did you figure out what type of control was appropriate? I'm curious too.