Trypan blue - (Mar/06/2013 )
Everybody, i need your help.
Then I need to use it in cell culture. Usually people use
How I can prepare that from Trypan blue dye 60% (302643)?
Thank you very much
You just registered to ask this question? I'm impressed
let's say you want 1ml of 0.4%
0.6xv1=0.004x1000ul =>v1= 6.6 ul, take this amount from the original bottle and top up to 1 ml with water.
i asked so many questions before this
this is my 3rd account since the other 2 one got problem so i cant longer used the account
I asked this because, at my place, no one use that trypan blue powder.
all of them used the ready made one, so I think the best is to ask people in Bioforum.
Thank you so much
I bought 2 bottle and it must be used, rather than waste the money
but...it is in powder form
doesn't matter, calculate everything based on w/v instead of v/v
Thank you moderator
What about a control? We work in a clinical lab and use TB to get a cell vialbilty of the bone marrow products after collection, before infusion etc. We are looking for a way to have controls to prove TB is working....perferably something we can make that kills half the cells so we can see both positive and negative. Any ideas?