EDTA bacterial cell lysate - (Jan/03/2010 )
Does anybody using EDTA to lysate bacterial cell ?
Could you please provide me the lysate buffer ?
i am using 2 buffers
1) Tris/Cl 20mM, EDTA 2.5mM, sucrose 20% (30mins on ice) 10ml for 1L bacterial culture
2) Tris/Cl 20mM, EDTA 2.5mM (30 mins on ice) 10ml for 1L bacterial culture
I will discard the (1) solution, and then centrifuge down the pellet, then add (2)
I found that almost 50% of the target protein was appeared in the (1)
what kind of bacteria?
aimikins on Jan 5 2010, 07:31 AM said: