cell culture media refrigeration - (Oct/25/2011 )
If DMEM a) with L-glutamine without L-glutamine is not refrigerated for 1-2 days, what effect will this have?
It is not shipped refridgerated....does that answer your question?????????
thanks for the replies. is there any harm associated with degraded l-glutamine?
Our media with L-glutamine in it comes shipped cold.
That said- 1 or 2 days at room temp should be fine (if you think about it, your media will be at 37C for that amount of time before you replace it).
I usually replenish L-glu after a month in the fridge.
If this is an ongoing concern of yours, you could consider either purchasing media with a stable l-glu substitute, or purchase media l-glu free and then keep frozen aliquots of l-glu to add just prior to using the media?