Sf9 cell suspension culture - (Jul/22/2012 )
we use Fernbach flasks instead of erlenmeyer flasks for insect culture.
Are Fernbach flasks made of polycarbonate?
Have you ever compared growing Sf9 cells in Fernbach and Erlenmeyer flasks?
Do you prevent the formatrion of the ring of cells by growing Sf9 cells in Fernbach flasks?
The formation of the ring is not due to the flask, but rather is a property of liquid/solid interfaces, where you get high surface tension of the liquid that results in objects being pulled towards the high tension. I doubt you can avoid it all other than by using roller bottles, and even these will have some cells being affected by a similar effect in some places in the bottle.
What you say sounds right.
However, don't you think that a flask made with a material with low tendency to adhesion (such as polycarbonate or, for the sake of argument, teflon) would prevent the formation of the ring of cells?
Potentially yes. However, in the overall scheme of things a few dead cells are not going to make a real difference to your experiments - they should be roughly the same in both controls and treatments.