normalization to protein content or cell number - (Mar/22/2012 )
I need to determine the amount of my compound in my treated cells, and I realize that some express their compounds being normalized to protein level or per cell number (e.g. per a million cell). I am wondering if there is a reason to favour one over the other? I am leaning towards expressing it per protein just because expressing per cell number by cell counting might probably give a greater variation as opposed to using bradford assay. But then I'd like to know if anyone else have insights regarding the topic.
Either should be fine. If your counting is accurate enough (or you have a machine to do the counting for you), I find that this is easier to compare to in terms of real usable numbers i.e. ones I can imagine/visualise.
Thank you Bob1!