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Break Cell Clumps - prepare single cells for injections (May/31/2011 )

Hallo!

I have murine cancer cells in culture and I use them to do injections in mice. I want to have single cells because it's very important to seed the same cell number. Do you know how I can achive that?

-science_rocks-

science_rocks on Tue May 31 12:26:51 2011 said:


Hallo!

I have murine cancer cells in culture and I use them to do injections in mice. I want to have single cells because it's very important to seed the same cell number. Do you know how I can achive that?

Hola, check if after trypsination they continue aggregated if yes try dislodge by pipetting up,down. Buena suerte

-protolder-