Good comprehensive resource for maintaining immortal cell lines? - (Mar/11/2009 )
Just looking for a reputable, preferably free, online resource to obtain information about protocols that support effective & responsible culturing/sub-culturing of immortal cell lines (i.e., J774s, etc.).
Pretty basic, but it is OK:
Otherwise I recommend R.I Freshney's Culture of Animal Cells. It is the best reference material that I have come across.