Cell culture medium contamination? - (Feb/16/2014 )
This is my first post in this forum. I would like to ask your advice, because I found particles floating up from the bottom of my medium bottles, when I stirred them.
I found them even in a medium that I completed 4 days ago and stored on +4°C! There is no other change in the media, the color is normal.
I started to incubate the media samples 4 days ago in the incubator.
So far I see
- fibrils (which I think, come from the FCS, because in the FCS they are also present)
- and there are larger floating, or sometimes attached particles, they look like coagulates of something similar.
There is nothing moving in the medium. The cells look like normal.
I am so nervous, because they are present in all media. Can the "coagulates" come from the FCS?
Can I save the media with sterile filtering?
Thanks a lot!
Yea, there is often particulate in FBS. You can sterile filter if you're concerned.