Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

CaCl2 for making competent cells - (May/05/2010 )

This is my first time trying to make competent cells ;) , and i'm going to adopt the protocol from Sambrook. the question is do I have to use CaCl2 and MgCl2 with molecular biology grade? or biochemical grade is okay? :unsure: thanks before

-boboey-

Biochemical grade would be sufficient. I think I autoclaved my cacal2 1M.
you can autoclave your glycerol as well, but I never did.

I use invitrogen's protocol for making TOP10

-Curtis-

biochemical grade would be sufficient i guess. I normally sterile filter my solutions. Good luck!

-biolab12-

biolab12 on May 10 2010, 09:50 PM said:

biochemical grade would be sufficient i guess. I normally sterile filter my solutions. Good luck!


Thank you Curits and Biolab12 for the answers, making me more confident :ph34r:
And Yes, we will surely sterile filter the solutions..

-boboey-