siRNA transfection of RS4-11 and MV-4-11 Cells - (Mar/20/2009 )
Has anyone successfully transfected either RS4-11 or MV-4-11 cells using siRNA with a efficiency of >85%? Right now I am having problems with electroporation....most of my cells die. I am following the protocols from Amaxa on these cell lines, but even they say that transfection efficiency is poor.
I would actually prefer to use some sort of cationic/lipid based transfection reagent. Has anyone tried lipofectamine 2000 or Hiperfect or something similar in these cell lines?
Also, has anyone tried using a lentiviral shRNA for gene knockdown in these cell lines?
check literature. you could also check with the technical support for any transfection reagent.