knockout mouse with increase cell size - (Feb/03/2009 )

I was looking in Pubmed and MGI for knockout mouse models

I want to find a gene that when knocked out, will stop (or reduce) cell proliferation, but the cells in the mouse will continue to enlarge, such that the volume of an individual cell in the KO will be bigger than the wildtype

E.g. in drosophila, I've seen knockdown of Cdk1 will generate this phenotype, but I couldn't find a KO with this phenotype

Does anyone know of one?

If so, please let me know the paper that describe it.

Thanks a lot

jiro_killua on Feb 3 2009, 08:34 PM said:

You should look for SKP2 knockout, the cells are double the size.

knockingout p27 in these mice reverts the size to normal.

Basically, all genes of this cell-cycle pathway have cell size relevance, so study SKP2, p27, CKS1 etc.

cellcounter on Feb 5 2009, 09:31 AM said:

Great, thanks a lot

Skp2 KO are smaller in overall size too

The most interesting and ideal model would be a mouse with normal size overall, the individual cell size are doubled, and the total number of cells were halved.

Is that possible? :>

jiro_killua on Feb 5 2009, 09:59 AM said:

I don't think that would be possible with mouse. It would not survive beyond organogenesis. Too complex a system. However, I may be wrong. With SKP2 too, it is mainly hepatocytes that show the phenotype if I remmber it correctly.

For your ideal conditions, it may be possible with drosophila larvae or C.elegans, or perhaps even xenopus or zebrafish embryos, but I would not expect such to happen in an adult mouse. I stand corrected if somebody knows this field better.