How long does it take for cells to reattach? - From balls to pancakes. (Jan/31/2011 )
Not that this affects my experiments, but I'm just curious if anyone know how long does it take for (your favorite) cells to re-attach after splitting?
I'm talking about from little balls to the pancakes shape.
I'm currently working with MCF-10a.
In my case (E14) they have differential attachment. The first ones to attach can do it in just 1 hour but the rest of them (I call them late bloomers) can attach in the following 6 hours.
It depends on the cell type and how transformed it is... some like HeLa only take a couple of hours, others like primary cells often take 12 hours or more.
most of the cells that I culture can attach within 4hr,
but I usually give them O/N,
anything that cannot attach during O/N incubation, I consider dead cells