cell adhesion in serum free condition - (Jun/21/2010 )
I need to do check proliferation of my cell lines in serum free condition. So I have two choices:
1) trypsinize cells and resuspend cells in 10%FBS medium, plate cells; change medium to serum free the next day;
2) after trypsinizing and resuspending in 10%FBS medium, centrifuge cells, aspirate 10%FBS medium; resuspend cells in serum free medium and plate cells at desired amount.
I would prefer choice 2) since washing and changing medium may cause loss of this specific cell line. But I don't know if cells can adhere to the plate when plated in serum free medium.
Any suggestions? Thanks!
By the way, it is LNCaP cells. Any suggestion?