Cytotoxicity assay for adherent cells exposed to particles - (May/18/2010 )
I am wondering if you can help me with finding an appropriate assay to study the cytotoxicity of particles. I am incubation my cells (adherent cells) with different doses of particles (nanoparticles and micron-sized particles) for different periods of time and wondering what is the best assay to determine the cytotoxicity of these particles. Would LDH assay work for me? I will have particles in the media, would that interfere with the assay? Any other recommendations?
Sayeh on May 18 2010, 11:24 PM said:
I use the MTS assay after I have exposed my cells to vesicles which may or may not be taken up by cells and have had no problems. The good thing I find is that I need to do my assay in a 24-well plate as the cells like some space around them and I can add the MTS then remove 100uL to put in a 96-well plate to read after the colour development. I can also leave the colour development to continue if it hasn't gone on long enough, there is no stop solution like MTT.
Another option might be Cell Titre Blue from Promega?