Culture Media for stem cells - (Sep/20/2010 )
Iīm isolating cancer stem cells from blood, and I try to culturing , I am using stem cell Pro, from invitrogen, but I donīt see any advance. Does anybody use the same method, or has any suggestion to prepare culture media, in order to expand the number of cells without diferentiation.
Do you use any cytokines at all? How are you isolating the stem cells from the blood?
There are plenty of companies out there that specialise in stem cell media and reagents, I guess it may be case of trying a few out and seeing if they work for you.
StemCell Technologies are quite a big one, but there are many more out there! You may want to try a serum free media or if you do use serum use serum that has been specially batch tested for stem cell culture.