Methanol concentration for cells? - (Jun/23/2014 )
I will solve a drug to use methanol for HT-29 cell line. Final concentration for methanol is 0,3 %. Is it toxic for the cell line? Can I use the drug at 0,3 % final concentration?
The answer is "Maybe" 0.3% will probably be fine, but it depends on the cell line a lot. Note that if the drug is soluble in methanol, it will also be soluble in ethanol, which might be less toxic.
if the drug is soluble in methanol, it will also be soluble in ethanol
Is it accurate? If Ok, I can use EtOH.
Methanol and Ethanol are very similar chemicals - the only difference is a CH3 group, so they behave in the same fashion as other members of the alcohol group.
I recommend a titration of your selected solvent and assess toxicity by yor
favorite assay. Alternatively, many drugs are available as water soluble salts or
in pharmaceutical encapsulations (eg., PEG or