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seperating cells and antibodies - Separating cells and antibodies using centrifugation (Oct/24/2009 )


At what speed should I run a centrifuge to separate cells secreting antibodies. Is 1000g at 5 mins enough to separate cells from the antibodies

Please reply...Also if you can send me the link where this procedure is mentioned, it will be helpful

thank you



Thank you for the tips, i will work on it. on Oct 26 2009, 03:00 PM said:

Pellet the cells by centrifugation (300xg for 5-10mins). Antibodies will be in the supernatant. Hope this helps.