N acetyl cysteine solution for cell culture - (Jul/02/2012 )
Hi! I need to dissolve N acetyl cysteine into cell culture media. I need it at 25 mM which is 40mg/mL.
How do I do this with minimal interference in ionic strength?
Or can i buy a stock solution of it, that is already at physiological pH?
Is it really difficult to dissolve?
If you got yours from Sigma it is soluble at 100 mg/ml in H2O (heating). I've used it some times and the medium pH drops a bit.
NAC is really easy to dissolve. You are using it at a fairly high concentration so you could dissolve it in your growth media. Did you mean to write mM and not uM for your working concentration? When I have used this in the past I found that mM concentrations kill cells.