how to separate organelles from mammalian cells - (Dec/11/2013 )
I want to get my target protein which is expressed in the CHO cells. With the help of GFP, I know my protein is localized in the nucleus membrane and ER membrane. Now I want to harvest my cells and separate the nucleus and ER from the cells. I have no experience on this aspect. And I want to get your advices. Could you tell me some references or some useful websites or protocols to help me deal with this?
Thank you very much.
look for cell fractionation protocols.