How to release the bacterial cells from filter paper? - (May/27/2014 )
I filtered my bacterial sample using vacuum filtration system. May I know is there any method can fully release out the bacterial that retain on the filter paper? Because I am going to proceed into next step which need to incubate the dried bacterial cells into nutrient broth.
why do you need to the whole bacteria for inoculation to NB? I had an experiment with filteration just for weighting the dried bacteria, but if you realy need to inoculate whole, I think you can put the paper in the broth flask or wash it in a sterile condition by a sterile pincer in the broth.
Why not place the entire filter in the nutrient medium?
Thanks for the suggestion. But do you think if I place/wash the filter paper in the nutrient medium will still have some remaining on the paper? Besides that, I was using pore size 0.45um to filter the bacteria culture, however I was noticed that the eluate contains the bacteria as well? Why does it happened? I thought filter paper 0.45um will retain all the bacterial cells? Thank you.
Standard bacterial filtration requires 0.20 or 0.22um, unless you are working with really big and/or filamentous guys that cause fast clogging of filters. In any case, with a fibrous material like filter paper will have a lot of bugs between the fibres. Most filtration membranes have a porous and/or fibrous surface, probably the only ones that are not like that are the Isopore filters, but they have a very limited filtration capacity due this. They are use for epifluorescence.