using cell lysate to run native gel? - (Feb/04/2010 )
If the protein I am interested in has a pI of 9.5 , can I use acidic native gel to separate it from other cellular protein? I think I will use western to detect it following the native gel. I know there is acetate acidic native gel, so shall I directly add the sample loading buffer (~pH4.3, containing acetate) to the cell lysate and run it?
Thanks alot for answering.
are you using a stacking gel?
if so, then you will want to adjust your sample to near the pH of the stacking gel, rather than that of the running gel.