Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (Aug/31/2010 )

I have some problem to get a negtive control of estrogen receptor beta for western blot? anybody have any suggestion? thanks.


if you look at this paper, they profiled all 49 nuclear receptor expressions in 51 human cancer cell lines. In the supplementary data you should find ERbeta also.

Expression profiling of nuclear receptors in the NCI60 cancer cell panel reveals receptor-drug and receptor-gene interactions.
Holbeck S, Chang J, Best AM, Bookout AL, Mangelsdorf DJ, Martinez ED.
Mol Endocrinol. 2010 Jun;24(6):1287-96. Epub 2010 Apr 7.
PMID: 20375240