primary cell isolation from CNS, how to remove myeline debris - (Oct/25/2011 )
I have a question for you,
I just started to do some culture of primary cell from mouse CNS,
but after dissociation/isolation, myeline debris is all over and make it almost impossible for me to count the # of cells.
Does anyone know some easy (and hopefully inexpensive way) to remove the debris?
I know there is myeline removal microbeads in market, but I prefer some other method since our lab does not use the microbeads system.
I prefer some density gradient protocol, so if anyone have any suggestion, please let me know.
Thanks in advance
Myelin debris is a pain for counting! What cells are you culturing and how old are the mice?