staph cell count - (Sep/23/2009 )

Hi,

I am doing an experiment that requires a similar cell number per ml (about 1 million). I have no experience with this bacteria and don't have a standard curve to correlate OD with cell number.

I can't use a serial dilution and plating system because I need this cell culture.

Can somebody help me figuring out how to come up with this number/ml?

Thanks a lot!!

-planktonica-

make a suspension in an appropriate liquid, count in haemocytometer, calculate cellnumber/mL, dilute (or add cells) so you get the cell number you need

-gebirgsziege-

See if you can find something on the web! If no data exist for your particular bacterium, use data from another of similar size. These things usually don´t have to be extremely accurate anyway

-Chimp-

Thanks for the suggestions!

I've been looking for things on the web without much success

-planktonica-

planktonica on Sep 23 2009, 06:51 PM said:

You could use a "system" like alamarblue to count and make a growth curve...and recalculate the total amount of numbers with a counting chamber (you do not know living from dead cells then... )

or do as gebirgsziege said.

-pito-

I'd not accept "something on the web." Generate your own data.

-eberthella-