Freezing cells, quick question - (May/29/2009 )
Just wondering. Do any of you freeze cells just in a -80 and keep them there for long term storage as opposed to -80 then transferring to a cryo vessel? Just curious if it makes that much of a difference.
It won't be long term storage in a -80C but you might still be able to store your cells for up a year or more as compared "long term" storage in liq N2 storage. Ice crystal will slowly grow in cells kept at -80C