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blood red cells - (Oct/20/2010 )

Hi guys! I'm a new member of this forum. It's amazing. I have a question for you.
My lab has a collection of dried human blood (nucleated red blood cells) samples on Whatman cards. We are interested in extracting total proteins to be used in ELISA assay. Do you know if there is some protocol for this? I'd like to ask you if it's a good idea or not.
Thank you very much

-hellashellas-

Hi and welcome to BioForum :D

I doubt you'll get protein from those samples, DNA is tough, but proteins ain't.

Clare

hellashellas on Wed Oct 20 10:07:04 2010 said:


Hi guys! I'm a new member of this forum. It's amazing. I have a question for you.
My lab has a collection of dried human blood (nucleated red blood cells) samples on Whatman cards. We are interested in extracting total proteins to be used in ELISA assay. Do you know if there is some protocol for this? I'd like to ask you if it's a good idea or not.
Thank you very much

-Clare-