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Trypsin Deactivation - (Sep/18/2011 )

Hi,

I want to learn if trypsin (after cell detachment) could be deactivated by DMEM only without serum??

What really stops trypsin? Ions? Proteins?

Thanks

-Selcen Celik-

a little googling and I found this: http://www.ncbi.nlm.nih.gov/pubmed/17211556

apparently it is due to specific trypsin inhibitors present in serum. I am sure you can buy these inhibitors separately if you really don't want serum in your sample but seru would be by far the easiest and cheapest way.

-philman-

Thank you for the reply :)

Yes I think so that serum is the easiest way for that...

-Selcen Celik-

Soy-bean trypsin inhibitor (try Sigma-Aldrich) is another way to do it, if you really have to exclude serum.

-bob1-

Hi Bob,

Thank you for your advice !
:)

-Selcen Celik-