Marker do discriminate cell cycle phases - G2 phase / Mitosis (Nov/20/2009 )

Hi.

When I treat cells with a compound I get an huge G2/M arrest, as assessed by flow cytometry. I want to check if the cells are arrested either in G2 or in M. How can I do this?

Is there any marker I can use to check this by immunofluorescence / flow cytometry?


Thanks for any answer.

You could look for microtubule formation for G2 and condensed chromosomes or similar for M

Thanks.

Do you know how to do this? I mean, are you referring to a simple DAPI staining?

I guess, fix and then antibody stain for microtubules. Condensed DNA, standard Giemsa staining should work. Both by light microscopy or fluorescence in the case of the antibody.