Is cultured cell reuse possible? - (Jun/03/2015 )
I know this is not a deep question, but I would like the following to be clarified. I have selected a colony of BL21(DE3) picked up from LB-agar plate to inoculate a liquid medium an hour ago. But I realized I screwed up and would like to do it. The LB-agar plate has been left in room temperature for an hour or so. Can I select another colony form that plate again? Could I put the LB-agar plate back into the incubator before inoculating again? Thank you in advance.
It should be fine, E. coli's growth at room temperature is only slowed, not completely inhibited.