Anybody ever use whole blood plasma serum stimulate cells? - (Aug/14/2009 )
I suspected that my interested chemistry must combine with something in the serum to stimulate target cells. So I want to use the blood serum of the mice + the chemistry to stimulate cells. Do anybody know how to do it or can give me some suggestions about the key words to find some papers doing this way? or recommand some paper to read?
Thank you very much, Henry.
-Henry Eub-
Henry Eub on Aug 14 2009, 12:19 PM said:
I suspected that my interested chemistry must combine with something in the serum to stimulate target cells. So I want to use the blood serum of the mice + the chemistry to stimulate cells. Do anybody know how to do it or can give me some suggestions about the key words to find some papers doing this way? or recommand some paper to read?
Thank you very much, Henry.
Thank you very much, Henry.
I guess you mean to stimulate your cells with a chemical compound in presence of mouse serum.
If so, you set up an experiment where you add various concentration of serum and various conc of your chemical compound to the cells. Include the negative controls where you have either not added any serum or not added chemical.
It would typically look like this in its simplest format:
24 hours Incubation
Cells alone
Cells + serum (at various concentrations)
Cells + compound (at various concentrations)
Cells + serum + compound (at various concentrations)
48 hours Incubation
Cells alone
Cells + serum (at various concentrations)
Cells + compound (at various concentrations)
Cells + serum + compound (at various concentrations)
72 hours Incubation
Cells alone
Cells + serum (at various concentrations)
Cells + compound (at various concentrations)
Cells + serum + compound (at various concentrations)
And then you need a readout assay for what happens to your cells when they are stimulated. This can be morphologic changes, proliferation/apoptosis changes, biochemical changes (enzymatic assays) or molecular level changes (specific RNA, protein levels or reporter constructs with luciferase, CAT etc).
-cellcounter-
cellcounter on Aug 14 2009, 01:39 PM said:
Henry Eub on Aug 14 2009, 12:19 PM said:
I suspected that my interested chemistry must combine with something in the serum to stimulate target cells. So I want to use the blood serum of the mice + the chemistry to stimulate cells. Do anybody know how to do it or can give me some suggestions about the key words to find some papers doing this way? or recommand some paper to read?
Thank you very much, Henry.
Thank you very much, Henry.
I guess you mean to stimulate your cells with a chemical compound in presence of mouse serum.
If so, you set up an experiment where you add various concentration of serum and various conc of your chemical compound to the cells. Include the negative controls where you have either not added any serum or not added chemical.
It would typically look like this in its simplest format:
24 hours Incubation
Cells alone
Cells + serum (at various concentrations)
Cells + compound (at various concentrations)
Cells + serum + compound (at various concentrations)
48 hours Incubation
Cells alone
Cells + serum (at various concentrations)
Cells + compound (at various concentrations)
Cells + serum + compound (at various concentrations)
72 hours Incubation
Cells alone
Cells + serum (at various concentrations)
Cells + compound (at various concentrations)
Cells + serum + compound (at various concentrations)
And then you need a readout assay for what happens to your cells when they are stimulated. This can be morphologic changes, proliferation/apoptosis changes, biochemical changes (enzymatic assays) or molecular level changes (specific RNA, protein levels or reporter constructs with luciferase, CAT etc).
Thank you so much. Is there any commercial mouse serum available or I have to prepare the serum myself? And I didn't find any paper. DOn't know how to set up a key word for pubmed.
I appreciate you help. Best! Henry
-Henry Eub-