RNA extraction from stained cells - (Aug/11/2010 )

Hi everyone,

I wanna extract RNA from cell that must be fixed before due to staining for cell sorting

But as we have known that most of fixative cause the low A260/230

And unfortunately, these RNA are required to perform the microarray analysis

Can anyone teach me how to preserve the integrity of RNA in fixed cells

Thanks for the answers

What are the cells fixed in? The fixative will determine the method and the effectiveness of the extraction.

I'm actually curious about this as well. We usually fix our cells in 3.7-4% PFA, wouldn't the actual staining process affect the integrity of the RNA as well?