Competent cells question: JM101 vs DH5alpha - (Apr/03/2012 )
I've been having difficulty tracking down E. coli JM101 in my lab's freezer inventory. I'm trying to replicate a published experiment that utilizes JM101 as a host for intermediate plasmids (pBluescript + cloned genes + resistance marker), and I'm wondering if I can just use DH5alpha as host to replicate these intermediate plasmids. Are there any issues with doing this?
I'm guessing it would be easier with DH5a. JM101 has an intact E. coli restriction system, which will reduce transformation efficiency for some constructs. It also is recA+, which may make some constructs less stable. Is this a paper from 1980?
It's a paper from 2003. But thanks for the info!