chemotherapeutic-resistant cell lines - (Jun/27/2013 )
hello there,
does anyone of you know how to produce chemotherapeutic-resistant cell lines?
thanks in advance
-moljul-
A basic procedure would be to take cells, treat with the drug at a non-lethal dose (say LD50) and select cells that are resistant. Another way would be to add a resistance gene by stable transfection.
-bob1-