looking for bovine skeletal muscle cells - (Sep/28/2009 )

does anyone know if there's any bovine skeletal muscle cell line that i can purchase?tQ

newbie101 on Sep 28 2009, 10:15 AM said:

Thymus, smooth muscle, kidney, spleen, adrenal cortex etc., but no skeletal muscle line that I know of.

Dr Teeth on Sep 28 2009, 07:42 AM said:

thats the thing..guess i have to make my own primary cultures then. do you know how long does a primary culture could last? i knew that its life span is much shorter compared to using cell lines

newbie101 on Oct 6 2009, 08:32 AM said:

A primary culture usually won't last through more than a handful of splittings, but are useful in that they tend to give more physiologic results since the lines aren't cancerous, polyploid, etc. However, if you wish to use this line for an extended period, you many be better off isolating your bovine skeletal muscle cells and transforming them into an immortalized culture line with SV40 or by other means.

thanx!

newbie101 on Oct 6 2009, 11:32 PM said:

I would imagine that the structure of a skeletal fibre (ie fused myoblasts) would make culturing extremely difficult. The closest I’ve seen to culturing is a student who dissected out individual fibres and kept them alive for a day or so for experimenting.