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Control vehicle treatment in cells - (Mar/16/2009 )

Hi,

I read about control vehicle treatment (like ethanol or DMSO) in cells, but never understood what exactly it means. Do i treat the same volume of ethanol or DMSO as present in my ligand? Please clarify.

-dsk-

exactly.
if you add 200 ÁL of your ligand, in DMSO (or PBS, water, ethanol...), then you need to do a control where you add 200ÁL of DMSO (or PBS, water, ethanol...) alone

-little mouse-

... to continue "little mouse's" post...

so that you can distinguish the effect of the treatment from the carrier/vehicle effect.

-bob1-