Protocol Online logo
Top : New Forum Archives (2009-): : Flow Cytometry

violet laser (405nm) and cell cycle staining with DAPI - (Apr/03/2012 )

i would like to know if it is possible to measure a cell cycle stained with DAPI with a violet (405nm) laser instead of a UV lamp? I know that concerning the excitation maximum of DAPI, UV would be much better than a violet laser, but since DAPI is very bright, are there people using the violet laser when a UV lamp is not available?
Thanks in advance.


I found DAPI very detectable under other filters too. I just had problem with UV because after a while under exposure the DAPI-stained nuclei turned green. It was like they were burning